PhalloidinAmanita muscaria mushroom. It can bind to fibrous actin, allowing the distribution of microfilaments in cells to be visualized through staining. Therefore, Rogetin fluorescent staining solution is widely used in biological research.

In various plant or animal cells, the labeled Phalloidin has similar affinity for large and small filaments, with an average of one penicillin molecule per actin subunit. Unlike antibodies, the affinity of penicillin for actin does not vary significantly between species.

✨ Why choose Phalloidin?

Precise locking: specific binding to F-microtubulin, not "entangled" with G-microtubulin

Signal MAX: 40 times fluorescence intensity increase, background noise eliminated with one button

Good news for lazy people: 30 minutes of rapid staining without the hassle of antibody incubation

All-rounder: Freeze/wax sections, living cells, organoids

Rainbow Team: 488/555/594 Three-color fluorescent marking is optional

Our product information:

Minimalist version of the experimental process:

fixed cells → permeation treatment → Phalloidin ring staining → slide observation

Detailed steps of the experimental process:

1.Cultivate good cell slides, gently suck out the culture medium, and wash the cells with PBS buffer for 3 times;

2.Fix in 4% polyformaldehyde at room temperature for 15-30 min;

Note: Methanol can destroy actin, so the fixative should not contain methanol, and avoid using formaldehyde solution containing methanol;

3. PBS wash cells 3 times, each time 5 min;

4. Add PBS containing 0.1% Triton X-100 to cover the cells, and permeate the cells at room temperature for 20 min;

5. Wash the cells with PBS 3 times, each time for 5 min;

6. Draw circles around the crawling sheet with a group pen. Take an appropriate amount of freshly prepared guaiacol peptide staining working solution (1xPBS diluted in a ratio of 1:50 to 1:200) and completely cover the cells, incubate at room temperature in the dark for 30-120 min.

Note: The staining volume can be adjusted according to the sample. During incubation, to avoid evaporation of the stain solution, place the cover slip in a sealed container. In addition, adding 1% BSA to the Phalloidin stolonifer staining working solution can effectively reduce the background; 7, wash cells with PBS 3 times, each time for 5 min;

8. Add ready-to-use DAPI staining solution to the slide to cover the cells, stain nuclei at room temperature for 5-10 min; wash the cells with PBS 3 times, each time for 1min (optional);

9. Add anti-fluorescence quenching film and seal the film;

10. Fluorescence microscope or laser confocal microscope observation results.