Intestinal organoids recapitulate tissue architecture and cell diversity, enabling studies of stem cell dynamics, symmetry breaking, and regeneration.

  Disease Modeling and Drug Screening: Organoids are used to model gastrointestinal diseases, screen drugs, and study host-pathogen interactions, including SARS-CoV-2 infection.

  Regenerative Medicine: Advances in transplantation and tissue engineering demonstrate the potential of organoids for repairing damaged intestinal tissue and treating conditions like short bowel syndrome.

  Technological Innovations: Integration with organ-on-chip, genome editing, and high-throughput screening platforms has expanded the utility of organoids.

  Comparative and Translational Research: Organoids from various species, including farm animals, provide insights into nutrition, infection, and genetic engineering.

  Clinical and Personalized Medicine: Patient-derived organoids serve as avatars for personalized therapy, disease prognosis, and biomarker discovery.

Normal colon organoid bright field image:

Normal colon organoid HE image:

Organoid delivery standards:

1) Deliver at least one P3 generation per case, with two tubes containing over 10^6 cells, either frozen or live;

2) Provide bright-field images (40X,100X, or 200X magnification);

3) Ensure the organs can be expanded and passaged at the client's laboratory.

Common experimental procedures for human colon organoids include:

1) Drug toxicity screening;

2) Gene editing experiments using shRNA and CRISPR/Cas9;

3) Tumor modeling studies;

4) Sequencing of various omics including DNA, RNA, proteomics, and metabolomics;

5) Single-cell sequencing;

6) Other experimental approaches.

Organoid culture protocol

With organoid technology at its core, the company focuses on major refractory tumors that severely impact human health. By developing tumor organoid culture media and constructing organoid models, combined with genomic and gene-editing technologies, we strive to deliver high-quality customized organoid technical services for clients. The company has established organoid culture systems for multiple human organ tissues including lung cancer, colon cancer, breast cancer, gastric cancer, mouse intestinal tissues, and liver cancer. We provide organoid culture media and model services to researchers and drug development companies, and have established strong partnerships with multiple Grade III-A hospitals to support preclinical studies of various new drugs.

A list of organoid culture kit can be provided:

 1. Human normal tissue organoid culture medium kit

 2. Human tumor organoid culture medium kit

 3. Mouse/Rat derived tumor organoid culture medium kit

 4. Mouse/Rat derived normal tissue organoid culture medium kit

 5. Large animal derived organoid culture medium kit

Cotact us:

Neorise Biotech Co., Ltd. 

Phone：15502157206

Email：info@neorise.net