Technology

Immunohistochemical

(IHC)

Immunofluorescence

(IF)

TSA mIHC

Color rendering method

DAB simple staining

DAB+ red color original bichrome

Primary and secondary staining was mixed

Multiple rounds of repeated fluorescence experiments

Type of primary antibody

compliant IHC

The species of the primary antibody should be different for multiple indicators

The species of multi-antibody is not limited

Type of secondary antibody

HRP-labeled secondary antibody

Different species of secondary antibody labeled with different fluorophores

HRP-labeled secondary antibody

Coloration effect

Only two indicators can be stained at the same time;

Double staining cannot observe the co-expression, and the two indicators need to be located differently;

Indicators cannot be observed in a single channel during double staining

The detection sensitivity is low

It is easy to quench

At the same time, the number of eggs marked is small

The detection sensitivity is high and the fluorescence signal is doubled

Strong anti-quenching

Multiple proteins (up to 7-10) are also tagged

Detection costs

If you need to stain multiple indicators, you need to cut many slices, which is not suitable for precious or small sample size samples

The species of antibody should be different, and the selection range is limited

Mixed staining is not conducive to exploring experimental conditions and increasing experimental costs

The species of antibody is not limited, and the selection range is wide

The experimental conditions can be adjusted at any time in the pre-experiment of multi-round staining