Sample type

Rupture of membranes

Antigen repair

Antibody elution

Matters need Attention

Parafin section

NA

EDTA9.0 Antigen repair solution (20X) (Cat:NR21003) 95℃  25min

Citric acid antigen repair solution (20X) (Cat：NR21002) 95℃ 25min

For some tissues that are prone to shedding, such as bone tissue, antibody elution can be used

Frozen section

Because frozen sections and cell crawling sheets are generally thick, triton-100 membrane breaking solution can dissolve the lipids on the cell membrane and nuclear membrane, and punch holes in the membrane to facilitate the entry of antibodies. If the index to be detected is membrane expression, the membrane can not be broken.

NA

Antibody eluent (Cat：NR21001) 37℃ for 5-15min, 1-2 times;

1. If the antibody binding is strong, the antibody elution solution and EDTA9.0 antigen repair solution (20X) (Cat:NR21003) or citric acid antigen repair solution (20X) (Cat：NR21002) are mixed for 60℃ use.

2. If the antibody elution time is too long, the antigen recognition may be reduced or DAPI nuclear staining may be weak. Pay attention to the control of antibody elution time

Therefore, TSA should be controlled within three standards for cell crawling/chromosomal sectioning/frozen sectioning

Citric acid antigen repair solution (20X) (Cat：NR21002) 60℃ 45min

Cell slide

Antibody eluent (Cat：NR21001) at 37℃ for 5-15min, 1-2 times