+86-15502157206
info@neorise.net
+86-15502157206
info@neorise.net
Tetraethylammonium salt signal amplification (TSA, Tyramide signal amplification) is a class of enzyme-based detection methods that use horseradish peroxidase (HRP) to label target proteins. Similar to the conventional DAB colorimetric method in immunohistochemistry, TSA also employs HRP-labeled secondary antibodies and has a corresponding "color development" step (HRP catalyzes the addition of tyramine fluorescein substrate to the reaction system, producing an active fluorescent substrate. This active substrate can covalently bind with tyrosine residues or other residues on the antigen, forming stable covalent conjugates of tyramine fluorescein on the sample. Subsequently, non-covalently bound primary antibody-secondary antibody-HRP complexes are washed away using thermal repair or antibody elution buffer. The process then repeats with another primary antibody-HRP secondary antibody for a second round of incubation, followed by a different tyramine fluorescein substrate. This cycle continues to achieve multiplex labeling. It is primarily used in immunohistochemical (IHC), immunofluorescent (IF), or in situ hybridization (ISH) experiments.
The components of this kit include:
Nova TYRplus fluorescent dye, TSA buffer, HRP-labeled secondary antibody, DAPI staining solution, antibody diluent, 3% hydrogen peroxide, and anti-fluorescence quenching mounting medium.
This kit is suitable for human and mouse samples.
For detailed information and operating procedures, please refer to the datasheet.
Experimental procedure for the reagent kit:
Kit storage temperature: Store at 4°C with a one-year validity period. For the storage requirements of kit components, please refer to the instructions. For detailed operating procedures, please consult the instruction manual.
Notes:
