Phalloidin is a toxin isolated from the lethal umbrella-shaped poisonous mushroom, which specifically binds to the bicyclic peptide of F-actin. Therefore, phalloidin labeled with fluorescent dyes can be conveniently used to study the distribution of F-actin. Phalloidin can stain F-actin at the nanomolar level. In various plant or animal cells, the labeled phalloidin exhibits similar affinity for both large and small filaments, with an average of one phalloidin molecule bound to each actin subunit. Unlike antibodies, the binding affinity of phalloidin to actin does not show significant variation across different species.
Kit composition:
|
Product Name |
Size (100T) |
Storage |
|
Phalloidin (488) |
100ul |
-20°C, protected from light |
|
Anti-Fluorescence Quenching Mounting Medium (Dry Type) (#NR21014) |
10mL |
4℃ |
|
10mL |
4℃ |
Workup solution preparation of phalloidin: Dilute with 1×PBS at a ratio of 1:50 to 1:200 prior to use. The dilution ratio may be adjusted based on actual staining effects. Prepare phalloidin working solution according to the required volume and use immediately after preparation.